Determination of Content and Entrapment Efficiency of Transferrin Modified Vincristine-Tetrandrine Lipo-somes
10.6039/j.issn.1001-0408.2016.22.04
- VernacularTitle:转铁蛋白修饰长春新碱-粉防己碱脂质体中主药含量及包封率的测定
- Author:
Xuetao LI
;
Wei TANG
;
Ying JIANG
;
Lan CHENG
- Publication Type:Journal Article
- Keywords:
Vincristine;
Tetrandrine;
Transferrin;
Liposomes;
Content determination;
Entrapment efficiency;
HPLC
- From:
China Pharmacy
2016;27(22):3034-3036
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method for the determination of main components content and entrapment efficiency of transferrin modified vincristine-tetrandrine liposomes. METHODS:HPLC method was adopted to determine the content of vin-cristine. The determination was performed on ELITE C18 column with mobile phase consisted of methanol-15%triethylamine (70∶30)at a flow rate of 1.0 ml/min. The determination wavelength was set at 297 nm and column temperature was 30 ℃. The sample size was 20 μl. Free drug was isolated from liposomes by dextran gel column chromatography,and entrapment efficiency was deter-mined. RESULTS:The linear range of vincristine was 160-1 600 μg/ml (r=0.999 8,n=5) with average recovery of 99.20%(RSD=0.26%,n=9). RSD of precision test was 0.070%(n=5). The average content of vincristine in liposomes was 0.790 mg/ml(RSD=0.15%,n=3),and the average entrapment efficiency was 85.94%(RSD=2.08%,n=3). CONCLUSIONS:The method is accurate,reliable,simple and rapid,and can be used for the determination of the content and entrapment efficiency of vincristine in transferrin modified vincristine-tetrandrine liposomes.
