Formulation Optimization and in vitro Release Property Study of Methotrexate Thermosensitive Hydrogels
10.6039/j.issn.1001-0408.2016.22.33
- VernacularTitle:甲氨蝶呤温敏凝胶的处方优化与体外释药特性研究
- Author:
Kefei JI
;
Hong LIU
;
Min GUO
;
Kangping XIONG
;
Mengmeng ZHOU
;
Si GUO
- Publication Type:Journal Article
- Keywords:
PLGA-PEG-PLGA;
Methotrexate;
Thermosensitive hydrogel;
Orthogonal test;
in vitro release
- From:
China Pharmacy
2016;27(22):3136-3138
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the formulation of Methotrexate thermosensitive hydrogels,and to study the in vitro re-lease property of it. METHODS:Taking PLGA-PEG-PLGA as matrix and mannitol as viscosity modifiers,Methotrexate thermosen-sitive hydrogels were prepared. Using the absolute value of the deviation of gelation temperature between 35 ℃,its formulation was optimized by orthogonal test using the concentrations of PLGA-PEG-PLGA,methotrexate and mannitol as factors. The dialysis bag method was used to investigate accumulative release rate of Methotrexate thermosensitive hydrogels and methotrexate solution within 336 h in vitro. RESULTS:The optimal formulation was as PLGA-PEG-PLGA of 20%,methotrexate of 0.10% and mannitol of 0.10%;gelation temperature were 35.1,35.0,35.0℃. The average drug-loading rate was more than 90%. 12 d accumulative re-lease rate was more than 90%(r=3). Methotrexate solution has been substantially completely relased within 240 h. CONCLU-SIONS:Methotrexate thermosensitive hydrogels with sustained-release are prepared successfully,and the preparation technology is simple and practical.
