Study on Percutaneous Absorption in vitro of 0.03%Tacrolimus Ointment
10.6039/j.issn.1001-0408.2016.22.09
- VernacularTitle:0.03％他克莫司软膏的体外透皮吸收研究
- Author:
Pengwei ZHANG
;
Wenqin SU
;
Shaoyi KUANG
;
Wei LIU
- Publication Type:Journal Article
- Keywords:
Tacrolimus ointment;
Percutaneous absorption in vitro;
Nude mice;
HPLC-MS
- From:
China Pharmacy
2016;27(22):3051-3053
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the percutaneous absorption of 0.03% Tacrolimus ointment,and to compare the difference of domestic test preparation and imported reference preparation. METHODS:Modified Franz diffusion cells were adopted in trans-dermal test in vitro;HPLC-MS method was used to determine permeation amount and rate in vitro,delay time of domestic test preparation and imported reference preparation 0.03%Tacrolimus ointment. RESULTS:24 h in vitro permeation amount of test and reference preparations were(3 907±1 191)and(3 896±1 064)ng/cm2;permeation rates were 186.7 and 182.9 ng/(cm2·h);de-lay time were 1.95 and 2.00 h,respectively(P>0.05). CONCLUSIONS:Test preparation shows good percutaneous property,and is similar to reference preparation in penetration absorbency through nude mice skin.
