Formulation Optimization of Indomethacin-loading Solid Lipid Nanoparticles by Box-Behnken Response Surface Methodology
10.6039/j.issn.1001-0408.2016.22.28
- VernacularTitle:Box-Behnken响应面法优化吲哚美辛固体脂质纳米粒处方
- Author:
Kai CHEN
;
Shou LI
;
Kai JIN
;
Jing ZHAO
;
Jifu HAO
- Publication Type:Journal Article
- Keywords:
Indomethacin;
Solid lipid nanoparticle;
Box-Behnken response surface methodology;
Formulation optimization
- From:
China Pharmacy
2016;27(22):3118-3121
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the formulation of indomethacin-loading solid lipid nanoparticle(SLN). METHODS:Us-ing indomethacin as model drug,glyceryl behenate as oil phase,poloxamer 188 and polyethylene glycol-12-hydroxystearic acid as emulsifier,with turbidity,entrapment efficiency and drug loading amount as index,Box-Behnken response surface methodology was used to optimize the amount of oil phase,emulsifier-oil phase ratio,drug-oil phase ratio. The physicochemical properties of SLNs were characterized by SEM and DSC. RESULTS:The optimal formulation was as follows as oil phase of 0.91%,emulsifier-oil ratio of 1∶1,drug-oil phase ratio of 1∶5. The turbidity,entrapment efficiency and drug loading amount of prepared nanoparticle were 1 025-1 030 NTU,98.94%-99.08%,2.43%-2.46%,respectively;particle size and polydispersity index(PDI)were 181.5-182.3 nm and 0.340-0.341(n=3). The results of DSC showed that indomethacin was not present in crystalline state dispersed into SLNs. CONCLUSIONS:The optimal formulation is screened successfully,and indomethacin-loaded SLNs have been prepared.
