Preparation and Determination of Entrapment Efficiency of Puerarin Polymeric Micelles
10.6039/j.issn.1001-0408.2016.22.29
- VernacularTitle:葛根素聚合物胶束的制备及包封率的测定
- Author:
Xiaoxing MA
;
Cuiyan HAN
;
Chang LIU
;
Dan ZHU
;
Xiaoyu SUI
;
Cheng YUAN
;
Haitao HUANG
- Publication Type:Journal Article
- Keywords:
Puerarin polymeric micelles;
Film dispersion method;
HPLC;
Centrifugal-millipore filter filtration method;
Entrap-ment efficiency
- From:
China Pharmacy
2016;27(22):3122-3124
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To prepare Puerarin polymeric micelles and establish a method to determine its entrapment efficiency. METHODS:Puerarin polymeric micelles were prepared by film dispersion method. The polymeric micelles and free drug were sepa-rated by centrifugal-millipore filter filtration method. The entrapment efficiency of puerarin polymeric micelles was determined by HPLC. Diamonsil C18(2)column was used with 1% citric acid solution-methanol(65∶35)at the flow rate of 1 ml/min. The detec-tion wavelength was set at 250 nm,and column temperature was room temperature. RESULTS:The prepared polymeric micelles were spherical and spherical-like in shape with a mean particle size of 54.12 nm,polydispersity index of 0.122,Zeta potential of -13.60 mV;the linear range of puerarin was 2-10μg/ml(R2=0.999 4)with average recovery rate of 99.2%(RSD=0.9%,n=3). The re-covery rate of free drug was 95.3%(RSD=1.7%,n=3). The mean entrapment efficiency and drug-loading amount of puerarin were(35.5±2.12)% and(0.3±0.07)%,respectively(n=3). CONCLUSIONS:Film dispersion method is suitable for the prepara-tion of Puerarin polymeric micelles. Established method is convenient,accurate and reliable for the content and entrapment efficien-cy determination of Puerarin polymeric micelles.
