Optimization of Enzymatic Extraction Technology of Polysaccharides from Schisandra chinensis by Central Composite Design-response Surface Methodology
10.6039/j.issn.1001-0408.2016.22.35
- VernacularTitle:星点设计-响应面法优化五味子多糖的酶提工艺
- Author:
Nannan ZHANG
;
Zhijun ZHU
;
Yachao JIANG
;
Hao DENG
;
Song GAO
;
Yingli LI
- Publication Type:Journal Article
- Keywords:
Schisandra chinensis polysaccharide;
Enzymatic extraction technology;
Central composite-response surface method-ology
- From:
China Pharmacy
2016;27(22):3142-3144
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize enzymatic extraction technology of polysaccharide from Schisandra chinensis. METH-ODS:Using pH value of enzymatic extraction solution,the amount of enzyme,extraction temperature as response factor,S. chi-nensis polysaccharide as response value,on the basis of single-factor experiments,3-factor,5-level central composite experimental design was adopted for the experiment. Validation test was also conducted. RESULTS:The optimal extraction technology was as pH value of 5.7,enzyme dosage of 1.3%,extraction temperature of 53 ℃. In validation test,the extraction rate of S. chinensis polysaccharide was 14.30%(RSD=1.84%,n=6). CONCLUSIONS:The optimized extraction technology is simple,reasonable and stable,and can be used for the extraction of polysaccharide from S. chinensis.
