Combination effect of AMD3100 and dexamethasone on the mobilization of hematopoietic stem cells
10.3969/j.issn.2095-4344.2016.36.005
- VernacularTitle:AMD3100联合地塞米松对造血干细胞的动员作用
- Author:
Beizhan YAN
;
Huimin MA
;
Cunquan KONG
;
Yu LIANG
;
Weiyan ZHU
;
Shuting JIANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(36):5351-5357
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The number of hematopoietic stem cel s in the peripheral blood is very low at normal physiological state. So it is critical to mobilize hematopoietic stem cel s from donor’s bone marrow into the peripheral blood.
OBJECTIVE:To study the combination effect of AMD3100 and dexamethasone on the mobilization of hematopoietic stem cel s in mice, thereby laying the foundation for clinical application.
METHODS:C57BL/6 mice were injected with AMD3100 and dexamethasone alone or in combination. Then, hematopoietic stem cel s in the peripheral blood and bone marrow were col ected. CD34+cel concentration in the peripheral blood and bone marrow was determined by flow cytometer and the content of leucocytes in the peripheral blood was counted by a normal method. The CFU-Mix colony formation ability of hematopoietic stem cel s was identified by cel colony forming assay.
RESULTS AND CONCLUSION:The concentration of CD34+cel s in the peripheral blood and bone marrow, the content of leukocytes in the peripheral blood and the CFU-Mix colony formation ability of hematopoietic stem cel s were al improved by both AMD3100 and dexamethasone and especial y their combined use. This indicates that both AMD3100 and dexamethasone could mobilize hematopoietic stem cel s to migrate from the bone marrow to the peripheral blood, and when used in combination, the mobilization effect is better than that of single drug.
