Variation of blood biochemical indices in liver cirrhosis rats after adipose-derived mesenchymal stem cell transplantation
10.3969/j.issn.2095-4344.2016.36.007
- VernacularTitle:脂肪间充质干细胞移植后肝硬化大鼠血生化指标的变化
- Author:
Haiyan HAN
;
Wenguang SONG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(36):5364-5370
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Stem cel transplantation is a promising treatment of advanced liver disease, and adipose-derived mesenchymal stem cel s have become another kind of popular cel s fol owing bone marrow mesenchymal stem cel s.
OBJECTIVE:To investigate the influence of adipose-derived mesenchymal stem cel transplantation on blood biochemical indices of liver cirrhosis rats.
METHODS:Sixty rats were equal y randomized into normal control, model and cel transplantation groups. Model rats of liver cirrhosis were made in the latter two groups through intragastric administration of carbon tetrachloride. One week after successful modeling, rats were given intraperitoneal injection of adipose-derived mesenchymal stem cel suspension in the cel transplantation group, and given normal saline in the other two groups.
RESULTS AND CONCLUSION:Compared with the normal control group, the model group showed a significant increase in the levels of alanine aminotransferase, aspartate aminotransferase, total bilirubin, total protein in liver tissues, serum level of malondialdehyde, 15-minute indocyanine green retention rate and degree of hepatic fibrosis, and a significant decrease in serum albumin level, serum albumin/globulin, levels of glutathione peroxidase and cyclic guanosine monophosphate in liver tissues. On the contrary, these indicators were al improved in the cel transplantation group compared with the model group.
Moreover, CM-Dil-positive cel s were visible in the liver tissue of rats undergoing adipose-derived mesenchymal stem cel transplantation. Al these findings indicate that adipose-derived mesenchymal stem cel transplantation can reduce liver cirrhosis in rats by acting on blood biochemistry levels.
