Study on the lmna gene knockdown in zebrafish embryo with morpholino oligonucleotides
10.3969/j.issn.1671-7856.2016.08.014
- VernacularTitle:利用吗啉代寡核苷酸技术下调早期斑马鱼胚胎lmna 基因的初步研究
- Author:
Feng LIU
;
Huimin HUANG
;
Zhihua WANG
;
Xijun WU
;
Zhixu HE
;
Liping SHU
- Publication Type:Journal Article
- Keywords:
Zebrafish;
lmna;
Gene knockdown;
Morpholino oligonucleotides;
Laminopathy
- From:
Chinese Journal of Comparative Medicine
2016;26(8):85-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective Lamins are the major components of nuclear lamina underneath the inner nuclear membrane (INM).Lamins express in most cells and are involved in the whole process of growth, also play a major role in cell stability and embryonic development.Mutant in human LMNA gene may lead to a series of disorders, which are similar to progeria or other aging-associate syndrome.In this study, we report a new lmna knockdown animal model generated in our laboratory in order to provide a useful tool for studying laminopathies.Methods Two plasmids tagged to zebrafish lmna gene were designed based on morpholino oligonucleotides technology.Co-microinjected the plasmids into zebrafish embryos to knockdown lmna gene.Imagining and western blot detection were used to identify the mutants.Results Two different proteins, Lamin A/C, were expressed in the zebrafish embryos.Two plasmids lmna-MO and lmna-EGFP-pCS 2 + were generated and co-microinjected into embryos.The results of imagining and western blot showed that the expression of lmna gene was downregulated in the zebrafish embryos.Conclusions Lamin A/C are expressed in zebrafish.lmna gene can be knocked down by the injection of lmna-MO and lmna-EGFP-pCS 2 +.This new animal model may be a powerful tool for study on laminopathies.
