Genetic monitoring and analysis of highly immunodeficient mice from different sources by microsatellite DNA markers
10.3969/j.issn.1671-7856.2016.08.015
- VernacularTitle:不同来源高度免疫缺陷小鼠微卫星 DNA 遗传检测的分析
- Author:
Tao FAN
;
Hong WANG
;
Jie WEI
;
Shuya ZHOU
;
Bingfei YUE
;
Baowen LI
- Publication Type:Journal Article
- Keywords:
Immunodeficient mice;
Microsatellite;
Genetic quality;
Mice
- From:
Chinese Journal of Comparative Medicine
2016;26(8):91-95
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test and analyze the genetic background of highly immunodeficient mice from different sources.Methods Four highly immunodeficient mouse strains from different sources of NOD background were collected. 30 microsatellite DNA sites were detected, and the genotype can be displayed by gel electrophoresis and STR scanning. Results 17 microsatellite sites exhibit polymorphism in 20 mice of the four groups.There were 30 homozygous loci in the mice of groups A and B, and heterozygous in the other two groups.The genetic distance is minimum between groups A and B, showing a higher genetic similarity.Conclusions The genetic backgrounds are different in highly immunodeficient mice from different sources.
