Expression of miR-139-5p in small cell lung cancer tissue and its clinical significance
10.13481/j.1671-587x.20160520
- VernacularTitle:miR-139-5p在小细胞肺癌组织中的表达及其临床意义
- Author:
Huanxin LIU
;
Guoxiang ZHANG
;
Linlang GUO
;
Songshan TAN
- Publication Type:Journal Article
- Keywords:
cancer,small cell lung;
real-time quantitative polymerase chain reaction;
miR-139-5p
- From:
Journal of Jilin University(Medicine Edition)
2016;42(5):942-948
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression of miR-139-5p in small cell lung cancer (SCLC)tissue and its clinical significance, and to clarify the role of miR-139-5p in the occurrence and development of SCLC. Methods:The biological function of miR-139-5p was examined by cell growth,apoptosis and cell cycle analysis. The expressions of miR-139-5p in 50 cases of cancer tissue and paracarcinoma normal tissue were examined by QRT-PCR.Combined with the clinical data,the role of miR-139-5p in clinic was anzlyzed.Results:The expression level of miR-139-5p in SCLC tumor tissue was lower than that in normal lung tissue (P <0.01).The expression level of miR-139-5p in the cells was un-regulated significantly after transferred miR-139-5p mimics (P <0.01).Compared with control group,the proliferation abilitiy of cells was reduced after up-regulating the expression of miR-139-5p (P < 0.01),the number of cells at G1 phase was increased (P < 0.05),and the cells were arrested at G0/G1 phase.The miR-139-5p expression was not associated with gender and age (P > 0.05).The expression level of miR-139-5p in the patient at LD stage was lower than that of the patients at ED stage (P <0.01).The expression level of miR-139-5p in the resistant patients was higher than that of the patients sensitive to chemotherappy (P <0.01).The expression level of miR-139-5p in the survival patients was lower than that in the death patients (P <0.01).Cox regression analysis indicated that miR-139-5p expression and disease stage were the independent prognostic factors for SCLC.Conclusion:miR-139-5p in participates in the occurrence and development of SCLC by inhibiting the cell proliferation,promoting apoptosis and inducing the cell cycle arrest;it may be used as a target gene to evaluate the prognosis of SCLC patients.
