Analysis on association between polymorphism of CD14 and IL-8 gene and susceptibility of necrotizing enterocolitis
10.13481/j.1671-587x.20160523
- VernacularTitle:CD14和IL-8基因多态性与新生儿坏死性小肠结肠炎易感性的关联性分析
- Author:
Jiayi TIAN
;
Tong ZHU
;
Jian WANG
;
Mingli FANG
;
Chaoying YAN
- Publication Type:Journal Article
- Keywords:
necrotizing enterocolitis;
cluster of differentiation 14;
interleukin-8;
single nucleotide polymorphism
- From:
Journal of Jilin University(Medicine Edition)
2016;42(5):958-962
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between the gene polymorphism cluster of differentiation 14 (CD14)-159C/T (rs2569190),and interleukin-8 (IL-8)-251A/ T (rs4073)and the susceptibility of necrotizing enterocolitis (NEC),to clarify the influencing factors of susceptibility of NEC and to provide genetics theory basis for the research on the pathogenesis of NEC. Methods:Total 28 newborns with NEC and 41 newborns without NEC were selected.The amplification of peripheral blood DNA was conducted by PCR.The genotypic and allelic frequencies of CD14-159C/T and IL-8-251A/T of the patients were detected by Sanger DNA sequencing method. The relationship between them and the susceptibility of NEC was studied.Results:The distribution of genotypic frequencies of CD14-159C/T and IL-8-251A/T was consistent with Hardy-Weinberg equilibrium (P >0.05).There were no significant differences of the allelic and genotypic frequencies of CD14-159C/T,or genotypic frequencies of IL-8-251A/T between two groups (P >0.05).While in NEC group,the T allelic frequency of IL-8-251A/T site was higher than that in control group (χ2 = 4.184, P = 0.041, OR = 2.14, 95% CI: 1.03 - 4.46 ). Conclusion:The polymorphism of CD14-159C/T is irrelevant to the pathogeny of NEC,but T allelic frequency of IL-8-251A/T site might be related to the susceptibility of NEC.So T allele in IL-8-251A/T may be one of the danger factors of NEC.
