Establishment of a zebrafish model of thrombosis and the intervention effect of Guanxinning tablet
10.3969/j.issn.1005-4847.2016.04.018
- VernacularTitle:斑马鱼血栓模型的建立与冠心宁片的干预作用
- Author:
Mulan WANG
;
Yongming PAN
;
Min JIN
;
Xiaoping XU
;
Dejun WANG
;
Quanxin MA
;
Minli CHEN
- Publication Type:Journal Article
- Keywords:
Guanxinning tablet;
Thrombosis model;
Zebrafish;
Anti-thrombotic effect;
Phenyl hydrazine;
Arachidonic acid;
Ponatinib
- From:
Acta Laboratorium Animalis Scientia Sinica
2016;24(4):432-438
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a zebrafish model of thrombosis induced by three kinds of inducers and observe the anti?thrombotic effect of a Chinese traditional medicine, Guanxinning tablet ( GXN) . Methods The zebrafish models of thrombosis was induced by using 1?5μmol/L phenyl hydrazine, 80μmol/L arachidonic acid and 5 mg/L ponatinib, re?spectively, and were treated with various concentration of GXN, clopidogrel or asprin. The thrombus in the tail vein was observed under microscope, Erythrocytes in the zebrafish heart were stained with o?dianisidine and the erythrocyte staining intensity was assessed with a NIS?Elements DTM image analyzer, and the anti?thrombolic effect of GXN was calculated. Results Venous thrombus was significantly increased and the staining intensities of erythrocytes in the heart were signifi?cantly decreased after induction by phenyl hydrazine, arachidonic acid or Ponatinib ( P <0?001 ) , respectively. At the same time, GXN showed an incresing anti?thrombolic effect in the zebrafish models (P<0?001) in a dose?effect manner, with a IC50 of GXN of 44?32 mg/L,138?5 mg/L and 459?5 mg/L, respectively. Conclusions The zebrafish models of thrombosis are successfully established by phenyl hydrazine, arachidonic acid or Ponatinib, respectively, by different for?mation mechanisms. GXN has been shown to have an anti?thrombosis effect, probably, by multiple target effects.
