Expression of Disabled-1 in human breast cancer cells and its role in cell cycle
10.13481/j.1671-587x.20160518
- VernacularTitle:Disabled-1在人乳腺癌细胞中的表达及其对细胞周期的影响
- Author:
Rangjuan CAO
;
Kai LI
;
Wanying XING
;
Yueshu WANG
;
Wei YU
;
Guangzhi WU
;
Shusen CUI
;
Qiang LI
- Publication Type:Journal Article
- Keywords:
Dab1;
human breast cancer cell;
cell cycle;
G1 phase inhibition
- From:
Journal of Jilin University(Medicine Edition)
2016;42(5):932-936
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expressions of Disabled-1 (Dab1 )in human breast epithelial cells and breast cancer cells,and to clarify its role in cell cycle.Methods:Real-time PCR was used to analyze the Dab1 mRNA expressions in breast epithelial cells MCF-10A and breast cancer cells MCF-7,BT-549,and MDA-MB-231. The Dab1 protein expressions in those cells were tested by Western blotting method. The BT-549 cells at logarithmic growth phase were divided into control,pKH3,and pKH3-Dab1 groups;the cell cycle was investigated by flow cytometry.Results:The Real-time PCR results showed that the Dab1 mRNA expression levels in MCF-7 cells (0.504 ± 0.037),BT-549 cells (0.302 ± 0.027),and MDA-MB-231 cells (0.330 ± 0.031 )were reduced compared with MCF-10A cells (0.998±0.020)(P <0.05).The Western blotting results showed that the Dab1 protein expression levels in breast cancer cells MCF-7 (0.134±0.014),BT-549 (0.076±0.01),and MDA-MB-231 (0.074±0.005)were reduced compared with MCF-10A cells (0.227±0.021)(P <0.05).Compared with control group and pKH3 group,the cell cycle in pKH3-Dab1 group was inhibited at G1 phase detected by flow cytometry analysis. Conclusion:The expression of Dab1 is down-regulated in breast cancer cells,and the over-expression of Dab1 can inhibit the cell cycle at G1 phase.
