Application of quantum dots immune fluorescent labelling in colorectal cancer tissues
10.3969/j.issn.1673-4130.2016.18.010
- VernacularTitle:量子点荧光标志结肠癌细胞及在其组织芯片的应用价值
- Author:
Fengping HE
;
Xin XU
;
Qingsong WU
;
Dingyun LI
;
Zhanzhong MA
;
Sheng TANG
;
Weidong YIN
;
Haitao GONG
;
Hengxian LIN
- Publication Type:Journal Article
- Keywords:
quantum dots fluorescent labelled;
K-ras;
MXRA5;
colorectal cancer
- From:
International Journal of Laboratory Medicine
2016;37(18):2531-2533
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate different antigens detected by a novel labelled reagent‐quantum dots(QDs) in the colorectal cancer tissues microarray(TMA) .Methods Depend on QDs streptavidin conjugate(QDs‐SA) combined specially with biotinylation IgG ,immune of luorescent histochemistry was utilized to examine expression of K‐ras ,matrix‐remodeling associated 5(MXRA5) proteins in the colorectal cancer TMA ,where the protein accurate location was observed .Results K‐ras ,matrix‐remodeling associ‐ated 5(MXRA5) proteins were high expressed in colorectal cancer tissue and located accurately in the cell membrane and nucleus of colorectal cancer cells ,respectively .Conclusion QDs exhibit excellent photostability ,broad emission spectrum and long fluorescence lifetime .Modified with streptavidin could accurately detect different protein locations in the colorectal cancer TMA .This is a novel approach for studying targeted imaging of colorectal cancer in vivo and vitro clinical diagnosis .
