Study on the Quality Standard for Maikang Mixture
10.6039/j.issn.1001-0408.2016.21.40
- VernacularTitle:脉康合剂的质量标准研究
- Author:
Lin SHEN
;
Jia QU
;
Yongyue SUN
- Publication Type:Journal Article
- Keywords:
TLC;
HPLC;
Schisandrin;
Quality control
- From:
China Pharmacy
2016;27(21):3000-3003
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the quality standard for Maikang mixture. METHODS:TLC was used for the qualitative identification of Paeonia lactiflora,Angelica sinensis,Ligusticum chuanxiong,Astragalus membranaceus,Schisandra chinensis and Ophiopogon japonicus. HPLC was used for the content determination of schisandrin:the column was Kromasil C18 with mobile phase of methanol-water (60∶40,V/V) at a flow rate of 1.0 ml/min,detection wavelength was 254 nm,column temperature was 40 ℃,and the infection volume was 20 μl. RESULTS:P. lactiflora,A. sinensis,L. chuanxiong,A. membranaceus,S. chinensis and O. japonicus showed clear spots and well separated. The linear range of schisandrin was 1-50 μg/ml (r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was 97.01%-98.58%(RSD=0.56%,n=6). CONCLU-SIONS:The established standard can be used for the quality control of Maikang mixture.
