Content Determination of Berberine Hydrochloride in Fufang Huangge Jiangtang Tablet by UPLC
10.6039/j.issn.1001-0408.2016.21.41
- VernacularTitle:UPLC法测定复方黄葛降糖片中盐酸小檗碱的含量
- Author:
Yuzhen YANG
- Publication Type:Journal Article
- Keywords:
Fufang huangge jiangtang tablet;
Berberine hydrochloride;
UPLC
- From:
China Pharmacy
2016;27(21):3004-3005
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of berberine hydrochloride in Fufang huangge ji-angtang tablet. METHODS:UPLC was performed on the column of Acquity UPLC BEH C18 with mobile phase of acetoni-trile-0.1% H3PO4(21∶79,V/V)at flow rate of 0.3 ml/min,the detection wavelength was 345 nm,column temperature was 30℃, and the volume injection was 1.0 μl. RESULTS:The linear range of berberine hydrochloride was 0.008 16-0.081 6 μg(r=0.999 9);RSDs of precision,stability and reproducibility were lower than 1%;average recovery was 97.05%-98.96%(RSD=0.66%,n=6). CONCLUSIONS: The method is simple,precise,stablity and reproducible,and can be used for the quality control of Fufang huangge jiangtang tablet.
