Simultaneous Determination of Chlorogenic Acid,Rutin and Isoquercitrin in Morus alba and Its Processed Products by HPLC

VernacularTitle:HPLC法同时测定桑叶药材及其炮制品中绿原酸、芦丁和异槲皮苷的含量
Author: Congmei CHENG ; Juhua MAO ; Le YU
Publication Type:Journal Article
Keywords: Morus alba; Processed products; Chlorogenic acid; Rutin; Isoquercitrin; HPLC
From: China Pharmacy 2016;27(21):2990-2992
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To establish a method for the simultaneous contents determination of chlorogenic acid,rutin and iso-quercitrin in Morus alba,fried M. alba and honeyed M. alba. METHODS:HPLC was performed on the column of Agilent Zorbax SB-C18 with mobile phase of acetonitrile-0.2% phosphoric acid(gradient elution)at a flow rate of 1.0 ml/min,detection wavelen-gth was 350 nm ,the column temperature was 30 ℃,and injection volume was 10 μl. RESULTS:The linear range was 8.20-82.01μg/ml for chlorogenic acid (r=0.999 9),2.76-27.60 μg/ml for rutin (r=0.999 9) and 4.74-47.39 μg/ml for isoquercitrin (r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 98.58%-100.91%(RSD=1.02%,n=6),99.19%-101.00%(RSD=0.82%,n=6) and 98.41%-101.51%(RSD=1.08%,n=6),respectively. CONCLU-SIONS:The method is simple with good stability and reproducibility,and can be used for the simultaneous determination of chloro-genic acid,rutin and isoquercitrin in different processed drugs of M. alba.