Involvement of PI3K/AKT and MAPK Pathways for TNF-alpha Production in SiHa Cervical Mucosal Epithelial Cells Infected with Trichomonas vaginalis.
10.3347/kjp.2015.53.4.371
- Author:
Jung Bo YANG
1
;
Juan Hua QUAN
;
Ye Eun KIM
;
Yun Ee RHEE
;
Byung Hyun KANG
;
In Wook CHOI
;
Guang Ho CHA
;
Jae Min YUK
;
Young Ha LEE
Author Information
1. Department of Obstetrics and Gynecology, Chungnam National University School of Medicine, Daejeon 301-131, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Trichomonas vaginalis;
SiHa cell;
PI3K/AKT;
MAPK;
TNF-alpha
- MeSH:
Cell Line;
Cervix Uteri/enzymology/metabolism/*parasitology;
Epithelial Cells/*enzymology/metabolism/parasitology;
Female;
Humans;
*MAP Kinase Signaling System;
Mucous Membrane/*enzymology/metabolism/parasitology;
Phosphatidylinositol 3-Kinases/genetics/*metabolism;
Proto-Oncogene Proteins c-akt/genetics/*metabolism;
Trichomonas Vaginitis/*enzymology/genetics/metabolism/parasitology;
Trichomonas vaginalis/*physiology;
Tumor Necrosis Factor-alpha/genetics/*metabolism
- From:The Korean Journal of Parasitology
2015;53(4):371-377
- CountryRepublic of Korea
- Language:English
-
Abstract:
Trichomonas vaginalis induces proinflammation in cervicovaginal mucosal epithelium. To investigate the signaling pathways in TNF-alpha production in cervical mucosal epithelium after T. vaginalis infection, the phosphorylation of PI3K/AKT and MAPK pathways were evaluated in T. vaginalis-infected SiHa cells in the presence and absence of specific inhibitors. T. vaginalis increased TNF-alpha production in SiHa cells, in a parasite burden-dependent and incubation time-dependent manner. In T. vaginalis-infected SiHa cells, AKT, ERK1/2, p38 MAPK, and JNK were phosphorylated from 1 hr after infection; however, the phosphorylation patterns were different from each other. After pretreatment with inhibitors of the PI3K/AKT and MAPK pathways, TNF-alpha production was significantly decreased compared to the control; however, TNF-alpha reduction patterns were different depending on the type of PI3K/MAPK inhibitors. TNF-alpha production was reduced in a dose-dependent manner by treatment with wortmannin and PD98059, whereas it was increased by SP600125. These data suggested that PI3K/AKT and MAPK signaling pathways are important in regulation of TNF-alpha production in cervical mucosal epithelial SiHa cells. However, activation patterns of each pathway were different from the types of PI3K/MAPK pathways.
