Simultaneous Determination of Gallic Acid and Ellagic Acid in Xihe Majun Ointment by HPLC
10.6039/j.issn.1001-0408.2016.18.33
- VernacularTitle:HPLC法同时测定西诃蜜膏中没食子酸和鞣花酸的含量
- Author:
Jianmei LI
;
Hoja MAMAT
;
Tursun XIRALI
- Publication Type:Journal Article
- Keywords:
Xihe majun ointment;
Gallic acid;
Ellagic acid;
HPLC
- From:
China Pharmacy
2016;27(18):2546-2548
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of gallic acid and ellagic acid in Xihe majun ointment. METHODS:HPLC method was performed on the column of X-Bridge C18 with mobile phase of methanol-acetoni-trile-0.2% Phosphoric acid solution (gradient elution) at a flow rate of 1.0 ml/min,detection wavelength was 254 nm,detection temperature was 30 ℃,and injection volume was 10 μl. RESULTS:The linear range was 0.132-0.792 μg for gallic acid (r=0.999 9) and 0.108 0-0.648 2 μg for ellagic acid (r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 3%;recoveries were 95.15%-99.69%(RSD=1.64%,n=9) and 97.24%-100.43%(RSD=1.24%,n=9). CONCLUSIONS:The method is simple,accurate and sensitive,and can be used for the content determination of gallic acid and ellagic acid in Xihe majun ointment.
