Simultaneous Determination of Lithospermic Acid and Rosmarinic Acid in Guanxin Danshen Capsule by UPLC
10.6039/j.issn.1001-0408.2016.18.38
- VernacularTitle:UPLC法同时测定冠心丹参胶囊中紫草酸和迷迭香酸的含量
- Author:
Fujun ZHANG
;
Dongxuan ZHANG
- Publication Type:Journal Article
- Keywords:
UPLC;
Guanxin danshen capsule;
Lithospermic acid;
Rosmarinic acid
- From:
China Pharmacy
2016;27(18):2560-2562
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of lithospermic acid and rosmarinic acid in Guanxin danshen capsule. METHODS:UPLC was performed on the column of ACQUITY UPLC-BEH C18 with mobile phase of 0.1% formic acid-methanol solution (gradient elution) at a flow rate of 0.3 ml/min,detection wavelength was 280 nm,column temperature was 30 ℃,and the injection volume was 5 μl. RESULTS:The linear range was 0.019-0.76 mg/ml for lithospermic ac-id(r=0.999 9)and 0.005-0.2 mg/ml for rosmarinic acid(r=0.999 7);RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 97.34%-101.24%(RSD=1.57%,n=6) and 98.30%-100.39%(RSD=0.86%,n=6). CONCLU-SIONS:The method is simple,accurate and reproducible,and can be used for the contents determination of lithospermic acid and rosmarinic acid in Guanxin danshen capsule.
