Determination and Comparison of the Content of Sodium Benzoate in Medicinal and Non-medicinal Grade by HPLC
10.6039/j.issn.1001-0408.2016.18.40
- VernacularTitle:HPLC法测定并比较药用级与非药用级苯甲酸钠的含量
- Author:
Gui SU
;
Yanming LIU
;
Haiyan LONG
;
Ying LI
;
Bin LIAO
- Publication Type:Journal Article
- Keywords:
HPLC;
Sodium benzoate;
Medicinal;
Non-medicinal;
Content;
Comparison
- From:
China Pharmacy
2016;27(18):2566-2569
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method to determine and compare the contents of sodium benzoate in medicinal(phar-maceutical excipients and active pharmaceutical ingredients) and non-medicinal (chemical reagents and food additives) grade. METHODS:HPLC was conducted for content determination,SPSS 18.0 software was adopted to compare the results. The column was Purospher STAR LP RP-18 endcapped with mobile phase of acetotrile-0.02% formic acid(adjusted pH to 4.0 with aqua ammo-nia)(30∶70,V/V)at a flow rate was 1.0 ml/min,the detection wavelength was 230 nm,column temperature was 35 ℃,and vol-ume injection was 20 μl. RESULTS:The linear range of sodium benzoate was 10.5-525.3 μg/ml(r=0.999 9);RSDs of precision, stability,reproducibility and durability tests were lower than 0.5%;recovery was 99.38%-101.26%(RSD=0.56%,n=9). The av-erage contents of sodium benzoate in medicinal and non-medicinal grade were between 99.400%-99.875%,but the average content of non-medicinal grade is lower than the medical grade. CONCLUSIONS:The method is accurate and simple with high specificity and good reproducibility,and can be used to determine and compare the content of sodium benzoate in medicinal and non-medici-nal grade.
