Simultaneous Determination of Caffeic Acid,Isoschaftoside and Schaftoside in Xiaoyan Lidan Tablet by RP-HPLC
10.6039/j.issn.1001-0408.2016.18.45
- VernacularTitle:RP-HPLC法同时测定消炎利胆片中咖啡酸、异夏佛塔苷、夏佛塔苷的含量
- Author:
Xiaomei ZHANG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Xiaoyan lidan tablet;
Caffeic acid;
Isoschaftoside;
Schaftoside;
Content determination
- From:
China Pharmacy
2016;27(18):2582-2584
- CountryChina
- Language:Chinese
-
Abstract:
ABSTARCT OBJECTIVE:To establish a method for the simultaneous determination of caffeic acid,isoschaftoside and schafto-side in Xiaoyan lidan tablet. METHODS:RP-HPLC was performed on the column of Phenomenex C18 with mobile phase of metha-nol- 0.1% phsphate acid(gradient elution)at flow rate of 0.8 ml/min,the detection wavelength was 334 nm,column temperature was 25 ℃,and the injection volume was 10 μl. RESULTS:The linear range was 0.10-2.81 μg for caffeic acid(r=0.999 9), 0.20-5.63 μg for isoschaftoside(r=0.999 9)and 0.10-2.63 μg for schaftoside(r=0.999 9);RSDs of precision,stability and repro-ducibility tests were lower than 3%;recoveries were 96.3%-100.4%(RSD=1.59%,n=9),97.3%-101.2%(RSD=1.28%,n=9) and 96.6%-100.6%(RSD=1.39%,n=9),respectively. CONCLUSIONS:The method is sensitive,accurate,reliable and reproduc-ible,and can be used for the simultaneous determination of caffeic acid,isoschaftoside and schaftoside in Xiaoyan lidan tablet the quality control of Xiaoyan lidan tablet.
