Simultaneous Determination of Three Active Compounds in Heye Granule by RP-HPLC
10.6039/j.issn.1001-0408.2016.18.28
- VernacularTitle:RP-HPLC法同时测定荷叶颗粒中3种有效成分的含量
- Author:
Mengjie LI
;
Suzhen LIU
;
Bin ZHOU
;
Zhenhua CHEN
- Publication Type:Journal Article
- Keywords:
Heye granule;
Rutin;
Quercetin;
Nuciferine;
RP-HPLC
- From:
China Pharmacy
2016;27(18):2532-2534
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of rutin,quercetin and nuciferine in Heye gran-ule. METHODS:HPLC was performed on the column of Hypersil ODS2 with mobile phase of acetonitrile-water(containing 0.3%phosphoric acid and 0.4% triethylamine)(gradient elution)at a flow rate of 1.0 ml/min,the detection wavelength was 256 nm,the column temperature was 25 ℃,and the injection volume was 10 μl. RESULTS:The linear range was 0.012-0.240 μg for rutin(r=0.999 9),0.010 4-0.208 μg for quercetin(r=0.999 9)and 0.015-0.300 μg for nuciferine(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 98.5%-101.3%(RSD=1.1%,n=6),99.1%-101.6%(RSD=1.0%, n=6)and 98.9%-101.7%(RSD=1.2%,n=6). CONCLUSIONS:The method is simple,accurate and reliable,and can provide ref-erence for quality control of Heye granule.
