Separation and Preparation of 5 Phenylpropanoids from Cinnamomum Cassia by Semi-preparative HPLC and Their Immunosuppressive Activity Investigation
- VernacularTitle:半制备高效液相色谱法同时分离制备肉桂中5种苯丙素类化合物及其免疫抑制活性考察
- Author:
Junfen ZENG
;
Jianwu LU
;
Hucheng ZHU
- Publication Type:Journal Article
- Keywords:
Semi-preparative HPLC;
Cinnamomum cassia;
Phenylpropanoids;
Immunosuppression
- From:
China Pharmacist
2016;19(9):1625-1628
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To simultaneously separate 5 phenylpropanoids from Cinnamomum cassia by semi-preparative HPLC, and explore their immunosuppressive activity. Methods:After extracted by ethanol, the ethyl acetate part of Cinnamomum cassia was isola-ted by semi-preparative HPLC. The separation was conducted on an Ultimate XB-C18 (250 mm × 10 mm, 5μm) semi-preparative chro-matographic column and the mobile phase was acetonitrile-water with gradient elution. The detection wavelength was 330 nm, and the flow rate was 2. 5 ml·min-1 . The sample volume was 0. 3 ml. MS and NMR spectroscopic analysis were used to determine the config-urations. A CCK-8 method was used to detect the immunosuppressive activity of phenylpropanoids. Results: Totally 5 phenylpro-panoids were separated from Cinnamomum cassia by the semi-preparative HPLC, and identified as erythro-guaiacylglycerol, (7R,8S)-syringoylglycerol, (7S,8S)-syringoylglycerol, 3-methoxyphenyl-acrylaldehyde and O-methoxy cinnamaldehyde. The inhibitory rates of T cells and B cells of the compound 3 was more than 20% at the concentration of 800μmol·L-1 . Conclusion:The method is conven-ient with good separation effect, which can simultaneously separate 5 phenylpropanoids from Cinnamomum cassia, and among them, the compound 3 shows immunosuppressive effect to some extent.
