Safty evaluation, migration and distribution of human bone marrow derived-mesenchymal stem cells in the cns of young macaca fascicularis
10.3969/j.issn.1671-7856.2016.09.002
- VernacularTitle:hBMSCs幼年食蟹猴脑内移植的安全评价、迁移及分布
- Author:
Jiamei LI
;
Hua ZHU
;
Zhigang YAO
;
Wei DENG
;
Qin LI
;
Chunmei MA
;
Chuan QIN
- Publication Type:Journal Article
- Keywords:
Human bone marrow derived-mesenchymal stem cells;
Macaca fascicularis;
Migration;
Distribution
- From:
Chinese Journal of Comparative Medicine
2016;26(9):7-12
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evalutate the safty of hBMSCs transpalntation and to observe their migration and distribution in the brain of young macaca fascicularis. To establish a new technology platform and theoretical basis for the treatment of central nervous system diseases in children. Methods Labelled hBMSCs were transplanted into the striatum of young macaca fascicularis. Brain sections were examined to evalutate the inflammatory reaction and immunological rejection of local injection sites by HE observation and immunohistochemical staining. Migration and distribution of transplanted?hBMSCs was observed by real?time fluorescence quantitative PCR of male DNA and fluorescence microscope. Results The results showed that the direct intracerebral injection of hBMSCs did not cause systemic symptoms in animals. There is no inflammatory reaction and immunological rejection was detected, and degeneration and necrosis of neural cells and proliferation of glial cells were absent in the local injection sites. The transplanted hBMSCs survived, and migrated into the brain after 4 weeks transplantation. Its migration and distribution have certain regularity and were overlapping between transplant recipients. In addtion, hBMSCs tended to extend rostrally into the forebrain and showed preference of migrating toward the blood vessels and below the ependyma. Conculsions Intracerebral transplantation of hBMSCs is safe. And hBMSCs can survive and migrate into the brain.
