Effect of Astragaloside on the Growth of Human Keloid Fibroblast
- VernacularTitle:黄芪总苷液对瘢痕疙瘩成纤维细胞生长作用研究
- Author:
Yu FU
;
Liang ZHANG
;
Jing YANG
;
Jing YANG
;
Yihua WEI
- Publication Type:Journal Article
- Keywords:
Astragaloside;
Human keloid fibroblasts;
TGF-β/Smad signal transduction pathway
- From:
China Pharmacist
2016;19(9):1644-1647
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the inhibition of astragaloside on the proliferation of human keloid fibroblasts. Methods: Com-pared with that of normal skin, the expression of transforming growth factor-β( TGF-β) and its transduction factors Smad in the human keloid fibroblasts was detected. The optimal concentration was screened by MTT after HFF-1 human skin fibroblast was infected with astragaloside at different concentrations. The mRNA expression of Smad2, Smad3, Smad4 and Smad7 in the fibroblasts was studied by using real-time. The protein expression of TGF-βRⅡ, Smad2, Smad3, Smad4 and Smad7 in the fibroblasts was detected by using Western blot. Results: Compared with that of normal skin tissue, the expression of Smad protein was significantly increased ( P <0. 05) in the human keloid fibroblasts, and there was no significant difference in the TGF-βRⅡ expression (P>0. 05). The optimal concentration of astragaloside was 0. 5μg·ml-1 . The expression level of Smad2 protein in the two groups was significantly increased, and the level of Smad3 expression was significantly decreased (P<0. 05). Conclusion:Astragaloside can inhibit the formation of fi-broblast possibly through Smad2 over-expression and Smad3 inhibition in the TGF-β/Smad signal transduction pathway.
