Uncertainty Evaluation on the Determination of 6-Methylthiopurine by LC-MS/MS
- VernacularTitle:LC-MS/MS法测定6-甲基巯嘌呤含量的不确定度评定
- Author:
Changlu WANG
;
Shenghui MEI
;
Xiaoqing GONG
;
Li YANG
;
Zhigang ZHAO
;
Xinghu ZHANG
- Publication Type:Journal Article
- Keywords:
Uncertainty evaluation;
6-Methylthiopurine;
LC-MS/MS;
Human erythrocyte hemolysate
- From:
China Pharmacist
2016;19(9):1651-1655
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the uncertainty in 6-methylthiopurine (6-MMP) determination by LC-MS/MS. Methods:The uncertainty sources during the whole process of 6-MMP determination were established and evaluated. The combined and expanded un-certainties were also calculated. Results:The expanded uncertainty at low (2 995 pmol) and high (140 900 pmol) concentration of 6-MMP was 275. 6 pmol and 11 396 pmol, respectively (P=95%, k=2). Conclusion: The uncertainty in 6-MMP determination by LC-MS/MS is mainly caused by recovery, matrix effect and sample preparation at low concentration, and by recovery and sample prep-aration at high concentration.
