Effect of 4 . 1N gene transfection on biological characteristics of human lung cancer cell line ;A549
10.3760/cma.j.issn.1006-9801.2016.09.003
- VernacularTitle:转染4.1N基因对人肺癌A549细胞株生物学行为的影响
- Author:
Juan WU
;
Xin LIU
;
Qiaozhen KANG
;
Ge JIN
- Publication Type:Journal Article
- Keywords:
Lung neoplasms;
Protein,4.1N;
Cell line,A549;
Neoplasm metastasis;
Neoplasm invasiveness
- From:
Cancer Research and Clinic
2016;28(9):586-589
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of 4.1N expression in lung cancer A549 cell line on cell proliferation, invasion and migration. Methods A549 cells were cultured in vitro and transfected with lipofectamine 2000 mediation. Three groups were employed: transfection with pEGFP-4.1N plasmid, pEGFP vector plasmid, and blank control, respectively. The mRNA and protein expression differences of 4.1N was examined by semi-quantitative RT-PCR and Western blot in every group after 48 h. The proliferation capability was determined by MTT assay. Invasion capability was evaluated by scratches, adhesion experiments and Transwell chamber model. Results After the transfection, the expression of 4.1N mRNA and protein in pEGFP-4.1N plasmid transfection group was significantly enhanced (P<0.05). The proliferation capability of A549 cells descended extremely (P<0.05). The migration and invasion capability of A549 cells in vitro decreased substantially (P<0.05). Conclusions Transfected with 4.1N gene can significantly increases the expression levels of 4.1N mRNA and protein in A549 cells which are highly metastatic in human. Cell behavior in vitro studies showed that 4.1N gene can inhibit the proliferation, adhesion, invasion and migration of A549 cells, which plays an important role in the metastasis of lung cancer and it may become a molecular marker for metastasis of lung cancer.
