Synergistic Effect of Fosfomycin Combined with Carbapenems against Drug-resistant Pseudomonas aerugi-nosa Isolated from Urinary Tract Infections in vitro
10.6039/j.issn.1001-0408.2016.20.09
- VernacularTitle:磷霉素联合碳青霉烯类抗尿路感染耐药铜绿假单胞菌的体外协同作用研究
- Author:
Fengjun SUN
;
Zhijian XIONG
;
Wei FENG
;
Yixuan SUN
;
Peiyuan XIA
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Fosfomycin;
Carbapenems;
Urinary tract infection;
Synergistic effect
- From:
China Pharmacy
2016;27(20):2765-2767
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate synergistic effect of carbapenems combined with fosfomycin(FOS)on carbapenems-re-sistant Pseudomonas aeruginosa isolates from urinary tract infections in vitro. METHODS:The minimum inhibitory concentration was detected using agar double dilution method. The fractional inhibitory concentration index was determined by checkerboard meth-od. The effect of carbapenems combined with FOS on biofilm of P. aeruginosa isolates was determined using 96 crystal violet stain-ing. RESULTS:12 strains of carbapenem-resistant P. aeruginosa isolates were highly sensitive to FOS and amikacin,and were com-pletely resistant to imipenem and meropenem. The combination of imipenem with FOS could induce a synergistic effect on 4 strains (33.3%);meropenem combined with FOS could induce a synergistic effect on 5 strains(41.7%);no antagonistic effect of carbap-enems combined with FOS appeared. FOS combined with carbapenems could inhibit the biofilm of carbapenems-resistant P. aerugi-nosa(P<0.05 or P<0.01). CONCLUSIONS:The combination of carbapenems with FOS possesses in vitro synergistic antibacteri-al effect on part of carbapenems-resistant P. aeruginosa isolates,the mechanism of which may be associated with inhibiting the bio-film.
