Optimization of Ultrahigh Pressure Extraction of Forsythoside from Forsythia Suspensa by Box-Behnken Experimental Design
- VernacularTitle:Box-Behnken 试验设计优化超高压提取连翘中的连翘酯苷A
- Author:
Chenli YE
;
Xiayan XU
;
Jiang LIU
;
Xiaocong WANG
;
Weiling CAO
- Publication Type:Journal Article
- Keywords:
Ultrahigh pressure extraction;
Box-Behnken experimental design;
Forsythoside
- From:
China Pharmacist
2016;19(9):1670-1674
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize the ultrahigh pressure extraction ( UPE) process for forsythoside from Forsythia suspensa by Box-Behnken experimental design. Methods:On the basis of single factor screening, a three-factor and three-level Box-Behnken ex-perimental design was employed with liquid/material ratio( X1 ) , extraction pressure ( X2 ) and extraction time ( X3 ) as the independent variables. The response variable was the extraction yield of forsythoside. Results:The optimal UPE conditions for forsythoside were as follows:the ratio of solvent to material (ml·g-1) was 70, the extraction pressure was 151 MPa, and the extraction time was 114 s. With the optimal extraction process, the extraction yield of forsythoside was 13. 15 mg·g-1 . Conclusion:As a novel extraction tech-nology for Chinese herbal medicines, UPE procedure has higher extraction yield, lower extraction temperature, shorter extraction time and less power consumption, which provides a brand-new method for the extraction of forsythoside from Forsythia suspensa.
