Optimization of the Extraction Technology of Nucleosides from Codonopsis pilosula by Orthogonal Test
10.6039/j.issn.1001-0408.2016.16.28
- VernacularTitle:正交试验优化潞党参药材中核苷类成分的提取工艺Δ
- Author:
Gaofeng WANG
- Publication Type:Journal Article
- Keywords:
Codonopsis pilosula;
Ultrasonic extraction;
Nucleoside;
Orthogonal test
- From:
China Pharmacy
2016;27(16):2254-2256
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the extraction technology of nucleoside from Codonopsis pilosula. METHODS:The ultra-sonic extraction was used to extract nucleoside from C. pilosula. The extraction technology was screened and optimized by single factor and orthogonal test with ethanol concentration (including water),extraction time,solid-liquid ratio and extraction times as factors using the total contents of cytidine,uridine,guanosine and adenosine as index. Validation test was conducted. RESULTS:The optimal extraction technology was that pure water was extraction solvent,extracting for 45 min,with material-liquid ratio of 1∶20 (g/ml),extracting for 2 times. Total amount of 4 kinds of nucleosides from in 100 g C. pilosula was (17.01 ± 0.005) mg (RSD=0.68%,n=3). CONCLUSIONS:Optimized extraction technology of nucleoside from C. pilosula is stable,feasible,rapid in operation and cost-saving.
