Simultaneous Determination of Three Kinds of Components in Compound Xiaozhi Suppository by HPLC
10.6039/j.issn.1001-0408.2016.15.41
- VernacularTitle:HPLC法同时测定复方消痔栓中3种成分的含量
- Author:
Guangcai QI
;
Jiangrui WU
;
Mimi ZHANG
;
Shengfei CUI
;
Zhenye LIU
- Publication Type:Journal Article
- Keywords:
Compound xiaozhi suppository;
Gallic acid;
Rhein;
Emodin;
HPLC
- From:
China Pharmacy
2016;27(15):2138-2140
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of gallic acid,rhein and emodin in Com-pound xiaozhi suppository. METHODS:HPLC was performed on the column of Kromasil C18 with mobile phase of 0.5% phosphor-ic acid-Acetonitrile solution(gradient elution)at a flow rate of 1.0 ml/min,the detection wavelength was 273 nm for gallic acid and 254 nm for rhein and emodin,the column temperature was 28 ℃,and the injection volume was 20 μl. RESULTS:The linear range was 0.296 4-14.82 μg/ml for gallic acid(r=0.999 6),0.215 0-10.75 μg/ml for rhein(r=0.999 9)and 0.307 2-15.36 μg/ml for emo-din(r=0.999 9);RSDs of precision,stability and reroducibility tests were lower than 3.0%;recoveries were 95.1%-97.2%(RSD=0.64%,n=6),95.4%-97.2%(RSD=0.42%,n=6) and 96.5%-99.4%(RSD=1.10%,n=6),respectively. CONCLU-SIONS:The method is simple and accurate,and can be used for the contents determination of gallic acid,rhein and emodin in Compound xiaozhi suppository.
