Content Determination of Ecdysterone in Different Parts of Radix serratulae by HPLC
10.6039/j.issn.1001-0408.2016.15.44
- VernacularTitle:HPLC法测定广升麻植株中不同部位蜕皮甾酮的含量
- Author:
Yuqin XU
;
Zhihui WANG
;
Ribao ZHOU
;
Xiangdan LIU
;
Zhaohui WANG
;
Xiaorong LIU
- Publication Type:Journal Article
- Keywords:
HPLC;
Ecdysterone;
Radix serratulae;
Different parts
- From:
China Pharmacy
2016;27(15):2147-2149
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of ecdysterone in different parts of Radix serratu-lae. METHODS:HPLC was performed on the column of ZORBAX Eclipse XDB-C18 with mobile phase of methanol-water at a flow rate of 1.0 ml/min(46∶54,V/V),detection wavelength was 248 nm,column temperature was 30℃,and the injection volume was 10 μl. Compare the contents of ecdysterone in different parts of R. serratulae. RESULTS:The linear range of ecdysterone was 0.12-1.21 μg;RSDs of precision,stability and reproducibility tests were lower than 1.5%;recovery was 98.4%-101.9%(RSD=1.64,n=6). The content of ecdysterone in roots was found at the highest level,followed by tubers,and lower in stems and leaves, and it was not detected in seeds. CONCLUSIONS:The method is simple and rapid with good accuracy and reproducibility,and suitable for the content determination of ecdysterone in different parts of R. serratulae. It is feasible to develop the medicinal parts of R. serratulae from roots to roots,flowers,tubers and buds on tubers.
