Simultaneous Determination of the Three Ingredients of Zedoray Turmeric Oil in Ruxiankang Injection by HPLC
10.6039/j.issn.1001-0408.2016.15.45
- VernacularTitle:HPLC法同时测定乳腺康注射液中莪术油的3种成分含量
- Author:
Qingbo ZHANG
;
Fuming ZHANG
;
Wenjing CAO
;
Xueyan BI
- Publication Type:Journal Article
- Keywords:
Ruxiankang injection;
Zedoray turmeric oil;
Curdione;
Germacrone;
Furanodiene;
Content determination
- From:
China Pharmacy
2016;27(15):2150-2152
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of curdione,germacrone and furanodiene of zedoray turmeric oil in Ruxiankang injection. METHODS:HPLC was performed on the column of Zorbax SB-C18 with mobile phase of acetonitrile-water (gradient elution) at a flow rate of 1.0 ml/min,detection wavelength was 216 nm,column temperature was 30℃,and the injection volume was 10 μl. RESULTS:The linear range was 60-480 μg/ml for curdione(r=0.999 0),40-320 μg/ml for germacrone(r=0.999 0)and 40-320 μg/ml(r=0.999 0);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 95.21%-99.89%(RSD=1.6%,n=6),102.33%-104.89%(RSD=1.0%,n=6)and 97.38%-99.06%(RSD=0.7%,n=6),respectively. CONCLUSIONS:The method is simple and accurate,and can be used for simultaneous contents deter-mination of curdione,germacrone and furanodiene of zedoray turmeric oil in Ruxiankang injection.
