Study on Protective Effect of Glycyrrhiza uralensis Polysaccharide on CCl4 Induced Acute Liver Injury in Mice and Its Mechanism
10.6039/j.issn.1001-0408.2016.10.08
- VernacularTitle:甘草多糖对CCl4诱导急性肝损伤模型小鼠的保护作用及其机制研究
- Author:
Dongxue CHEN
;
Liang CHEN
- Publication Type:Journal Article
- Keywords:
Glycyrrhiza uralensis polysaccharide;
Acute liver injury;
Antioxidation;
Free radicals;
CCl4;
Mice
- From:
China Pharmacy
2016;27(10):1322-1325
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the protective effect of Glycyrrhiza uralensis polysaccharide (GUP) on CCl4 induced acute liver injury in mice and its mechanism. METHODS:60 ICR mice were randomly divided into normal control group (normal sa-line),model group(normal saline),bifendate group(positive drug,100 mg/kg)and GUP high-dose,medium-dose and low-dose groups (400,200 and 100 mg/kg). They were given relevant medicine intragastrically once a day. 15 d later,acute liver injury model was induced by intraperitoneal injection of CCl4 in those groups except for normal control group,and liver index was deter-mined. The levels of AST and ALT in serum,the NOS,NO,SOD,GSH-Px and MDA levels in liver tissues were detected. RE-SULTS:Compared with normal control group,the liver index,the levels of AST and ALT in serum and NOS,NO and MDA in liver tissue increased in model group,while SOD and GSH-Px levels in liver tissue decreased(P<0.05);liver cells became swell-ing,degeneration and necrosis,showed obvious inflammatory injury. Compared with model group,the liver index of GUP groups decreased,there was statistical significance in GUP high-dose medium-dose groups (P<0.05);the levels of AST and ALT in se-rum and NOS,NO and MDA in liver tissue of mice decreased in GUP groups,while the level of SOD and GSH-Px in liver tissue increased(P<0.05);liver cellular swelling,degeneration and necrosis relieved,and pathological injury had been improved. CON-CLUSIONS:GUP has a certain protective effect on CCl4-induced acute liver injury in mice and its protective effect may be related to antioxidation,decrease of NOS and NO levels,and reduction of the production of free radicals.
