Study on Spectrum-effect Relationship of Antipyretic Effect of Radix Bupleuri Injection
10.6039/j.issn.1001-0408.2016.10.13
- VernacularTitle:柴胡注射液解热作用的谱-效关系研究
- Author:
Chunmeng SU
;
Jiaxi TANG
;
Yang LUO
;
Chaoxia WANG
;
Tingting LI
- Publication Type:Journal Article
- Keywords:
Radix Bupleuri injection;
Fingerprint;
Antipyretic effect;
Spectrum-effect relationship;
Rat
- From:
China Pharmacy
2016;27(10):1340-1343
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the spectrum-effect relationship of the antipyretic effect of Radix Bupleuri injection. METH-ODS:HPLC method was used to establish the fingerprint of Radix Bupleuri injection. The antipyretic effect of 10 batches of Radix Bupleuri injection on fever rats induced by dried yeast were determined respectively. The fingerprint peaks were screened,along with the temperature value of different time points(0,10,15,30,40,55,70 min). The principal components were extracted by principal component method and the indirect relationship between the fingerprint and antipyretic effect was analyzed,depending on the principal component correlation coefficient matrix. RESULTS:There were 39 common peaks in fingerprint(similarity>0.85), No. 8,12,14,19,26,31,34,35 and 39 common peaks with large peak area were included in the study. Four principal compo-nents were extracted by principal component analysis(87% of total variant). First principal component showed that the active com-ponents of the 12th peak may be related to the antipyretic effect of 6 to 13 hours. The second principal component showed that the active components of the 26th peak may be related to the antipyretic effect of 0.5 to 5 hours. The third principal component showed that the similar effect of the active components could be caused by 34th,35th and 39th peaks. The fourth principal component sug-gested that there were some similarities between the 14th and the 31st peaks. CONCLUSIONS:Radix Bupleuri injection have obvi-ous improvement for fever rats. There is certain corresponding relation between HPLC fingerprint and antipyretic effect of Radix Bu-pleuri injection .
