Quality Evaluation of Solid Lipid Nanoparticle of the Skin Extract of Bufobufo gargarizans
10.6039/j.issn.1001-0408.2016.10.31
- VernacularTitle:蟾皮提取物固体脂质纳米粒的质量评价
- Author:
Lei ZHANG
;
Ziping YAN
;
Li ZHANG
- Publication Type:Journal Article
- Keywords:
Skin extract of Bufobufo gargarizans;
Solid lipid nanoparticle;
Cinobufagin;
Resibufogenin;
Encapsulation efficien-cy;
Drug-loading amount;
Stability
- From:
China Pharmacy
2016;27(10):1400-1403
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the quality of solid lipid nanoparticle of the skin extract of Bufobufo gargarzans. METH-ODS:The morphology of solid lipid nanoparticle of the skin extract of B. gargarzans was observed by TEM. The particle size was determined by laser scattering particle size analyzer. The contents of cinobufagin and resibufogenin, encapsulation efficiency, drug-loading amount and accumulative release rate of cinobufagin were determined by HPLC. The stability of nanoparticle was in-vestigated within 24 h at 60,25 and 4 ℃. RESULTS:The solid lipid nanoparticle of the skin extract of B. gargarzans were uni-form in particle size and showed round and spheroidicity shape;average particle size was(138.5±4.2)nm,The encapsulation effi-ciency of cinobufagin and resibufogenin were 90.60% and 91.51%,and drug-loading amount were 35.82% and 44.15%. The accu-mulative release rate of cinobufagin was 50%at 4 h and reached 88%at 48 h,which was in line with Weibull equation(r=0.9438). Under 3 kinds of temperature conditions,encapsulation efficiency decreased gradually as the holding time of nanoparticle pro-longed;the decrease degree was the smallest at 4 ℃.CONCLUSIONS:The quality evaluation results of solid lipid nanoparticle of the skin extract of B. gargarzans are in line with the standard,and prepared nanoparticles show sustained-release effects and should be kept under low temperature.
