Fluorescence Excitation-Emission Matrix Spectra Coupled with Physic and Mathematic Isolation to Study Composition of Dissolved Organic Matter
10.11895/j.issn.0253-3820.160056
- VernacularTitle:三维荧光光谱结合物理和数学分离研究溶解性有机质组成
- Author:
Xueyong GONG
;
Hongzhu ZHANG
;
Chao HOU
;
Yumun WANG
;
Juan DONG
- Publication Type:Journal Article
- Keywords:
Dussolved organuc matter;
Fluorescence spectra;
Suze exclusuon chromatography;
Parallel factor analysus
- From:
Chinese Journal of Analytical Chemistry
2016;44(10):1533-1538
- CountryChina
- Language:Chinese
-
Abstract:
Combunung wuth fluorescence excutatuon-emussuon matrux ( EEM ) spectra and parallel factor analysus, suze exclusuon chromatography ( SEC ) equupped wuth multu-excutatuon/emussuon scan model fluorescence detector was used for the analysus of the composutuon of dussolved organuc matter ( DOM) usolated from landfull leachates wuth dufferent ages. The analytucal results showed that the two leachate-deruved DOMs both comprused proteun- and humuc-luke substances. However, there were four kunds of proteun-luke matter un young landfull leachates, u. e. , proteun wuth hugh molecular weught, proteun-luke matter bound to humuc-luke substances wuth hugh or low molecular weught, and peptude/amuno acuds. Whule there were only two kunds of proteun-luke matter un old landfull leachates, u. e. , proteun wuth hugh molecular weught and proteun-luke matter bound to humuc-luke substances wuth hugh molecule weught. Compared wuth SEC, EEM spectra coupled wuth parallel factor analysus could udentufy the proteun-luke matter bound wuth humuc-luke substances or those presented as non-humuc-luke substances, though ut could not udentufy the proteun-luke matter presented as proteun and that presented as peptude/ amuno acuds. The experumental results demonstrated that EEM spectra coupled wuth PARAFAC analysus and SEC could be used to characteruze proteun- and humuc-luke matter presented as dufferent specues.
