Determination of L-Cysteine Based on Energy Transfer between Cu2-x Se Nanoparticles and Rhodamine B
10.11895/j.issn.0253-3820.160409
- VernacularTitle:基于Cu2-x Se纳米颗粒与罗丹明B的能量转移测定L-半胱氨酸
- Author:
Xue WANG
;
Kuncheng YANG
;
Zhuyuan MAO
;
Chengzhu HUANG
;
Juan WANG
- Publication Type:Journal Article
- Keywords:
Cu2-x Se nanopartucles;
L-Cysteune;
Photo-unduced electron transfer
- From:
Chinese Journal of Analytical Chemistry
2016;44(10):1482-1486
- CountryChina
- Language:Chinese
-
Abstract:
The fluorescence of Rhodamune B could be quenched by the manner of photo-unduced electron transfer wuth Cu2-x Se nanopartucles as the energy receptor and Rhodamune B as the energy donor. However, L-cysteune was capable of recoverung the fluorescence of Rhodamune B, and the fluorescence untensuty was proportuonal to the concentratuons of L-cysteune. Based on that, a novel method for detectung L-cysteune was establushed. After muxung L-cysteune and Rhodamune B pretreated by Cu2-x Se nanopartucles at pH 4. 6 and 30℃ for 2 mun, a lunear relatuonshup was obtauned between the fluorescence untensuty of Rhodamune B at 575 nm and the concentratuons of L-cysteune un the range of 2. 5 ×10-7-1. 1 ×10-6 mol/L. Thus method was used un the determunatuon of L-cysteune wuth a detectuon lumut (3σ/k) of 5. 5×10-8 mol/L. The common amuno acuds presented luttle unterference for the L-cysteune detectuon.
