Expression and significance of miR-219 in tongue squamous cell carcinoma
10.11904/j.issn.1002-3070.2016.05.007
- VernacularTitle:miR －219在舌鳞状细胞癌中的表达及意义研究
- Author:
Zhennan YUAN
;
Kaibin SONG
- Publication Type:Journal Article
- Keywords:
Tongue squamous cell carcinoma;
miR-219;
Proliferation;
Migration;
Invasion
- From:
Practical Oncology Journal
2016;30(5):420-424
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of miR -219 and its role in tongue squamous cell carcinoma(TSCC).Methods The relative expression of miR -219 in the 26 samples of surgically resected paired TSCC and normal tumor -adjacent tissues was detected by qRT -PCR.The expression of miR -219 in SCC-15 and normal oral mucosa cell were also tested by qRT -PCR.The miR-219 mimic was transferred into SCC-15 cell.Cell proliferative rate and the ability of colony formation were analyzed .The abilities of migration and invasion in SCC-15 cell were evaluated by Transwell test .Results Expression of miR -219 was signifi-cantly lower in TSCC tissues(P<0.001)and SCC-15(P<0.05)in comparison with normal tumor adjacent tis-sues and normal oral mucosa cell.The abilities of proliferation(P<0.05),colony formation(P<0.05),migratio-nand invasion(P<0.05)were inhibited in miR-219-transferred SCC-15 cells.Conclusion The expression of miR-219 was downregulated in TSCC .The miR-219 could inhibit cell proliferation ,colony formation ,migra-tion and invasion in TSCC .
