miR-150 deletion affects breeding and hematologic parameters of mice
10.3969/j.issn.1000-484X.2016.10.001
- VernacularTitle:miR-150基因缺失对小鼠繁殖和血液学指标的影响
- Author:
Quanhui ZHENG
;
Feng TIAN
;
Wenting LI
;
Qiuru ZHANG
;
Shan WANG
- Publication Type:Journal Article
- Keywords:
miR-150;
Gene deletion;
Mice;
Breeding;
Hematology
- From:
Chinese Journal of Immunology
2016;32(10):1409-1412
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of miR-150 gene deletion on the breeding and hematologic parameters of mice. Methods:The nest litter size,wean rate and weight changes of miR-150 knock out (miR-150ko) and C57BL/6J mice were com-pared. The hematology indexes were analyzed by automated blood cell counter, the serum biochemical parameters were analyzed by automatic biochemical analyzer. Results:The nest litter size and wean rate of miR-150ko mice were significantly decreased compared with that of C57BL/6J mice. The number of total white blood cells,intermediate cells,neutrophils,and the percentage of neutrophils and intermediate cells were significantly increased in miR-150ko mice compared with that of C57BL/6J mice. However,the number and per-centage of platelets and lymphocytes decreased significantly in miR-150ko mice. In addition,the levels of serum glucose and TC were in-creased significantly in miR-150ko mice compared with that of C57BL/6J mice. Conclusion: miR-150 gene deletion impairs the breeding and has complex impact on hematologic parameters of mice.
