Analysis on characteristics of fifteen short tandem repeat genetic polymorphisms in Yunnan Han population
10.3969/j.issn.1000-484X.2016.10.005
- VernacularTitle:云南汉族15个短串联重复序列基因座遗传多态性特征及分析
- Author:
Chanchan JIN
;
Jing HE
;
Lei WANG
;
Peng CHEN
;
Jiqing YANG
;
Xiuling LI
;
Jie SU
;
Baosheng ZHU
- Publication Type:Journal Article
- Keywords:
Short tandem repeat;
Genetic polymorphism;
Allele frequency;
Heterozygosity;
Forensic medicine
- From:
Chinese Journal of Immunology
2016;32(10):1428-1436
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To research on the genetic polymorphism distributions of fifteen short tandem repeat ( STR ) loci (D8S1179,D21S11,D7S820,CSF1PO,D3S1358,TH01,D13S317,D16S539,D2S1338,D19S433,VWA,TPOX,D18S51,D5S818, FGA) in Han race of Yunnan. Methods:A total of 313 specimens were collected from the unrelated individuals in Yunnan Han popu-lation. Genome DNA was extracted and amplified by multiplex PCR technique,the PCR products were analyzed by ABI-3130 genetic analyzer capillary electrophoresis detection, collected statistics of each STR loci genotypic frequency, and carried out the Hardy-Weinberg Genetic balance test. Results: No significant deviation from the Hardy-Weinberg Equilibrium was observed ( P>0. 05 ) , the heterozygosity of the fifteen STR loci in Yunnan Han population were found to be 0. 636-0. 901, Probability match was 0. 034-0. 220. Discrimination power of signal STR loci was 0. 780-0. 966, power of paternity exclusion was 0. 336-0. 797, polymorphism information content was 0. 555-0. 860,the combined accumulation discrimination power and exclusion probability for the 15 STR loci in Yunnan Han population were determined to be more than 0. 999 999 99 and 0. 999 998 408. The allele frequency of the 15 STR loci had a similarity compared with other areas in China,but also had a slight regional differences. Conclusion: The 15 STR loci( D8S1179, D21S11,D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818, FGA ) demonstrate high genetic polymorphism in Yunnan Han population, they have a high forensic science application value in paternity testing and individual identification.
