Effection of human umbilical cord blood stem cell transplantation on serum of rabbits with type 2 diabetes mellitus
10.3969/j.issn.1000-484X.2016.10.008
- VernacularTitle:人脐带血干细胞移植对2型糖尿病家兔血清学的影响
- Author:
Haixia DING
;
Fujun WANG
;
Bei LIU
;
Ning SHI
;
Yaping DU
;
Huiqing QI
;
Juan DING
- Publication Type:Journal Article
- Keywords:
Human umbilical cord blood stem cell;
Type 2 diabetes mellitus;
Rabbits;
Serology
- From:
Chinese Journal of Immunology
2016;32(10):1446-1449
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the changes of blood glucose,insulin and dipeptidyl peptidase-Ⅳ(DPP-Ⅳ/CD26)on type 2 diabetes mellitus in rabbits after HUCBSC( human umbilical cord blood stem cells) transplantation. Methods:18 rabbits were randomly divided into normal control group (6 rats,Group C) and diabetic model group (12 rats). After preparation model of type 2 diabetes,and 6 rats of them were treated with HUCBSC ( CD45+,CD34-) transplantation by ear vein transfusion ( Group A) ,and 6 rats were treated with PBS(Group B). All three groups of rabbits were fed for 4 weeks,and the blood glucose was monitored every day,and the level of blood insulin and DPP-IV/CD26 were measured every week. Results:The negative expression rate of CD34 in HUCBSC was 96. 5%. The positive expression rate of CD45 in HUCBSC was 100%. Compared with non transplantation group,the blood glucose and DPP-IV/CD26 in the umbilical cord blood stem cell transplantation group were gradually decreased,and insulin level was gradually increased, the difference was statistically significant (P<0. 01). Conclusion:HUCBSC were round or oval,with adherent growth,HUCBSC trans-plantation can significantly reduce blood glucose, increase insulin secretion, reduce the level of DPP-IV/CD26, the immunological phenotype of HUCBSC was CD45+,CD34-,thus providing a new theoretical basis for the clinical treatment of diabetes and its complica-tions.
