Effect of Gemcitabine on viability and apoptosis of non-small cell lung cancer HCC827 in vitro
10.3969/j.issn.1000-484X.2016.10.009
- VernacularTitle:吉西他滨对人非小细胞肺癌HCC827活性和凋亡的影响
- Author:
Jingjing ZHANG
;
Qingwei ZHAO
;
Jingrui YANG
;
Juhong DUAN
;
Haichuan YU
- Publication Type:Journal Article
- Keywords:
Cell apoptosis;
Human non-small cell lung cancer HCC827 cell;
Gemcitabine;
Bcl-2 protein
- From:
Chinese Journal of Immunology
2016;32(10):1450-1453
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Gemcitabine ( GEM) on the viability and apoptosis of non-small cell lung cancer HCC827 in vitro. Methods:The cell viability,apoptosis and cell cycle of HCC827 cells induced by Gemcitabine were detected with cell counting kit-8 assay (CCK-8),Annexin V-FITC/PI staining and flow cytometry. The expression of Bcl-2 protein of cells treated with GEM was examined by Western blot assay. Results: There was significant inhibition effect on HCC827 cells treated with 0. 1-1 000 ng/ml of GEM,which can promote the occurrence of HCC827 cell apoptosis and arrest cell in the S phrase. The apoptosis induced by GEM was accompanied with the down regulation of Bcl-2 protein. Conclusion: GEM can inhibit the cell viability and induce the HCC827 cell apoptosis and S phrase arrest. Its cell dead type was apoptosis,which was related with the expression of Bcl-2 protein.
