Enforced expression Foxp3 enhances proliferation and tumorigeneity of lung cancer cell
10.3969/j.issn.1000-484X.2016.10.016
- VernacularTitle:Foxp3对肺癌细胞增殖及成瘤的影响
- Author:
Ruimin LIU
;
Weixia CHAO
;
Shaolong WANG
;
Mingli WANG
;
Caiyun JIA
;
Huiling BAI
;
Yuanfang MA
- Publication Type:Journal Article
- Keywords:
Foxp3;
Overexpression;
Proliferation;
Tumorgeneity
- From:
Chinese Journal of Immunology
2016;32(10):1481-1484
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the effects of enforced expression of Foxp3 in lung cancer cell with regards to proliferation and tumorgeneity. Methods: A stable subline NCIH-hFoxp3 was established by liopfectamin-mediated pcDNA plasmid transfection carrying exogenous hFoxp3. The growth curve and secrection of IL-8 and IL-10 of NCIH-hFoxp3 were evaluated using MTT and ELISA, respectively. The in vivo tumorigeneity was assessed as well by inoculation of NCIH-hFoxp3 subcutaneously in nude mice. Results:Lung cancer cell NCIH-hFoxp3 with enforced expression of Foxp3 proliferated slowly but exihited increased in vivo tumorgeneity compared with corresponding control subline. In addition,increased expression of hFoxp3 in NCIH-hFoxp3 augmented secretion and at-tenuated secretion of IL-8 and IL-10,respectively. Conclusion:Increased expression of Foxp3 may promote progression of lung cancer cell by change of cellular microenvironment and evasion of immune surveillance.
