Expression and significance of CD4+CD45 RO+memory T cell in peripheral blood of patients with Hashimoto's thyroiditis
10.3969/j.issn.1000-484X.2016.10.025
- VernacularTitle:桥本氏甲状腺炎患者外周血中 CD4+CD45 RO+记忆性 T细胞的表达及意义
- Author:
Qianqian MA
;
Qiuhua LIANG
;
Lin SUN
;
Zhengjun ZHANG
;
Shipeng YU
- Publication Type:Journal Article
- Keywords:
Hashimoto's thyroiditis;
Memory T cell;
CD4+CD45RO+
- From:
Chinese Journal of Immunology
2016;32(10):1527-1531
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role CD4+CD45RO+memory T cells in the pathogenesis of Hashimoto's thyroiditis (HT) by detecting the percentages of CD4+CD45RO+ memory T cells in peripheral blood mononuclear cells in peripheral blood of newly diagnosed HT patients. Methods:53HT patients and 43 matched healthy controls (HC) were included in this study. According to the thyroid functions,HT patients were divided into euthyroid subset(HT-A,n =15) ,subclinical hypothyroidism(HT-B,n=14) and overt hypothyroidism subset (HT-C,n=24). The percentages of CD4+CD45RO+memory T cells in PBMCs,as well as the level of serum IFN-γ and IL-17,and thyroid functions,and the titers of thyroid-specific autoantibodies (TPOAb,TgAb) were respectively detected by flow cytometry,ELISA,and ECLIA. Results:The percentages of CD4+CD45RO+ memory T cells in PBMCs,as well as the level of serum IFN-γ and IL-17,the titers of TPOAb,TgAb were all significantly higher than that in HC(P<0. 01). Bivariate correlation revealed that the percentages of CD4+CD45RO+ memory T cells positively correlated with the level of serum IFN-γ,TPOAb and TgAb(P<0. 01,P=0. 015,P<0. 01) in HT patients. Conclusion:The significant increase of CD4+CD45RO+memory T cells in peripheral blood of patients with HT suggested a role of CD4+CD45RO+ memory T cells in the pathogenesis of this disease.
