Overexpression of von Hippel-Lindau protein inhibits the growth of colon cancer and liver metastasis in mice

VernacularTitle:过表达 von Hippel －Lindau 蛋白抑制小鼠结肠癌的生长及肝转移的研究
Author: Hui MIAO ; Longtu ZHU ; Zhilin XU ; Zheng ZHAO ; Jizhou WANG
Publication Type:Journal Article
Keywords: von Hippel-Lindau protein; Colon cancer; Liver metastasis
From: Practical Oncology Journal 2016;30(5):385-390
CountryChina
Language:Chinese
Abstract: Objective To investigate whether overexpression of VHL can inhibit the growth and metasta-sis of colon cancer .Methods Adenovirus vectors containing VHL were used to overexpress VHL protein in vitro and in vivo.The proliferation,apoptosis and migration of colon cancer cells CT 26 were examined in vitro.The ex-pressions of related proteins were detected by Western blot analysis .Subcutaneous and liver metastasis of colon cancer models were established and the therapeutic effects were evaluated .Results Overexpression of VHL pro-tein inhibited the proliferation and invasion of CT26,and promoted the apoptosis of CT26 via the regulation of COX-2,Bcl-2 and MMP-9.Overexpression of VHL protein inhibited the growth of subcutaneous colon cancer and liver metastasis in vivo .Conclusion Overexpression of VHL protien can inhibit the growth and metastasis of colon cancer ,and enhance the apoptosis of colon cancer ,indicating that VHL can be used as a potential target for colon cancer therapy .