Study on the Effect of Vitamin E Against Methylmercury-induced Neurotoxicity in Cultured Spinal Motor Neurons .
10.11637/kjpa.1996.9.1.109
- Author:
Yeun Tai CHUNG
;
Seung Taeck PARK
- Publication Type:Original Article
- Keywords:
NSC-19;
Antioxidant;
Cell culture;
Methylmercury
- MeSH:
Animals;
Antioxidants;
Cell Count;
Cell Culture Techniques;
Cell Line;
Cell Survival;
Intermediate Filaments;
Mice;
Motor Neurons*;
Neuritis;
Neurons;
Neuroprotective Agents;
Vitamin E*;
Vitamins*
- From:Korean Journal of Physical Anthropology
1996;9(1):109-116
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In order to investigate the neurotoxic effect of methylmercury (MM) on cultured mouse spinal motoneuron cell line, NSC-19, neurotoxic effect of MM was evaluated by MTT assay after neurons were incubated with various concentrations of methylmercuric chloride (MMC) for 24 hours. In addition, neuroprotective effect of vitamin E against MMC-induced neurotoxicity was examined by MTT assay and neurofilament enzymeimmunoassay (EIA) in these cultures. The results were as follows : 1. MTT50 value was a concentration of 20µM methylmercuric chloride. 2. Methylmercuric chloride was toxic on cultured spinal motor neurons, NSC-19 cells in a time-and dose-dependent manner by severe decrease of cell viability. 3. Methylmercuric chloride induced the decrease of cell number and the loss of neuritis on these cultures. 4. Vitamin E remarkably increased the viabilily of cultured neurons damaged by methylmercury-induced neurotoxicity at a concentration of 250µM vitamin E. From above the results, it is suggested that methylmercury induces severe toxic effect on cultured mouse spinal motor neurons, NSC-19 cells, and the selective antioxidants such as vitamin E are effective in the neurotoxicity induced by methylmercury in these cultures.
