Study on Effects of Podophyllotoxin Derivative QW-83 on Human Cervical Cancer HeLa Cell Apoptosis and Its Mechanism
10.6039/j.issn.1001-0408.2016.07.09
- VernacularTitle:鬼臼毒素衍生物QW-83对人宫颈癌HeLa细胞凋亡的影响及其机制研究
- Author:
Ling LENG
;
Chenguang GAO
;
Hong CHEN
;
Cong NIU
;
Bo CAO
- Publication Type:Journal Article
- Keywords:
Podophyllotoxin derivative;
QW-83;
Human cervical cancer HeLa cell;
Antitumor activity;
Apoptosis-related gene;
Etoposide
- From:
China Pharmacy
2016;27(7):892-895
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of podophyllotoxin derivative QW-83 on human cervical cancer HeLa cell apopto-sis and its mechanism. METHODS:After treated with 0(negative control),0.01,0.1,1 and 10 μmol/L QW-83 and positive drug etoposide(VP-16)for 48 h,proliferation inhibition rate and IC50 of HeLa cell were determined by MTT assay. The morphological changes of HeLa cell were observed by Hochest 33342 staining after treated with QW-83 [0(negative control),2.5,5,10μmol/L] for 48 h;flow cytometry was used to detect apoptosis rate;semi quantitative RT-PCR was adopted to detect the expression of apop-tosis related gene P53,Bax,Casepase-3,Casepase-8,Casepase-9 and Bcl-2 mRNA. RESULTS:Compared with negative control, 1,10 μmol/L VP-16 and QW-83 had obvious proliferation inhibition effect on HeLa cells (P<0.05 or P<0.01),and IC50 were (5.11±0.43)μmol/L and(4.96±0.54)μmol/L. Hochest 33342 staining results showed QW-83 could obviously induce cells apopto-sis and nuclear pyknosis. Flow cytometry showed QW-83 could increase apoptosis rate in concentration-dependent manner,being 16.89%-62.56%. RT-PCR showed mRNA expression of P53,Bax,Caspase-3,Casepase-8 and Casepase-9,Bcl-2/Bax increased, while mRNA expression of Bcl-2 decreased after treated with QW-83(P<0.05). CONCLUSIONS:Podophyllotoxin derivative QW-83 can induce HeLa cell apoptosis,and its mechanism may be associated with regulate mRNA expression of apoptosis related gene.
